Surface film formation in vitro by infant and therapeutic surfactants: role of surfactant protein B.

ABSTRACT:

Background

Pulmonary surfactant provides an alveolar surface-active film that is critical for normal lung function. Our objective was to determine in vitro film formation properties of therapeutic and infant surfactants and the influence of surfactant protein (SP)-B content.

Methods

We used a multiwell fluorescent assay measuring maximum phospholipid surface accumulation (Max), phospholipid concentration required for half-maximal film formation (½Max), and time for maximal accumulation (tMax).

Results

Among five therapeutic surfactants, calfactant (highest SP-B content) had film formation values similar to natural surfactant, and addition of SP-B to beractant (lowest SP-B) normalized its Max value. Addition of budesonide to calfactant did not adversely affect film for

SUBMITTER: Danhaive O 

PROVIDER: S-EPMC4391810 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature