Dataset Information

Species-specific Standardisation of Licorice by Metabolomic Profiling of Flavanones and Chalcones.

ABSTRACT:

Introduction

Major phenolics from licorice roots (Glycyrrhiza sp.) are glycosides of the flavanone liquiritigenin (F) and its 2′-hydroxychalcone isomer, isoliquiritigenin (C). As the F and C contents fluctuate between batches of licorice, both quality control and standardisation of its preparations become complex tasks.

Objective

To characterise the F and C metabolome in extracts from Glycyrrhiza glabra L. and Glycyrrhiza uralensis Fisch. ex DC. by addressing their composition in major F–C pairs and defining the total F:C proportion.

Material and methods

Three types of extracts from DNA-authenticated samples were analysed by a validated UHPLC/UV method to quantify major F and C glycosides. Each extract was characterised by the identity of major F–C pairs and the proportion of Fs among all quantified Fs:Cs.

Results

The F and C compositions and proportions were found to be constant for all extracts from a Glycyrrhiza species. All G. uralensis extracts contained up to 2.5 more Fs than G. glabra extracts. Major F–C pairs were B-ring glycosidated in G. uralensis, and A-/B-ring apiosyl-glucosidated in the G. glabra extracts. The F:C proportion was found to be linked to the glycosidation site: the more B-ring F-C glycosides were present, the higher was the final F:C proportion in the extract. These results enable the chemical differentiation of extracts from G. uralensis and G. glabra, which are characterised by total F:C proportions of 8.37:1.63 and 7.18:2.82, respectively.

Conclusion

Extracts from G. glabra and G. uralensis can be differentiated by their respective F and C compositions and proportions, which are both useful for further standardisation of licorice botanicals.

SUBMITTER: Simmler C

PROVIDER: S-EPMC4391967 | biostudies-literature | 2014 Jul-Aug

REPOSITORIES: biostudies-literature

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Publications

Species-specific Standardisation of Licorice by Metabolomic Profiling of Flavanones and Chalcones.

Simmler Charlotte C Jones Tristesse T Anderson Jeffrey R JR Nikolić Dejan C DC van Breemen Richard B RB Soejarto Djaja D DD Chen Shao-Nong SN Pauli Guido F GF

Phytochemical analysis : PCA 20131113 4

<h4>Introduction</h4>Major phenolics from licorice roots (Glycyrrhiza sp.) are glycosides of the flavanone liquiritigenin (F) and its 2′-hydroxychalcone isomer, isoliquiritigenin (C). As the F and C contents fluctuate between batches of licorice, both quality control and standardisation of its preparations become complex tasks.<h4>Objective</h4>To characterise the F and C metabolome in extracts from Glycyrrhiza glabra L. and Glycyrrhiza uralensis Fisch. ex DC. by addressing their composition in ...[more]

PMID: 25859589

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Dataset Information

Species-specific Standardisation of Licorice by Metabolomic Profiling of Flavanones and Chalcones.

Introduction

Objective

Material and methods

Results

Conclusion

Publications

Species-specific Standardisation of Licorice by Metabolomic Profiling of Flavanones and Chalcones.

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