Decitabine increases fetal hemoglobin in Papio anubis by increasing γ-globin gene transcription.

ABSTRACT:

Objective

The mechanism responsible for increased fetal hemoglobin levels following decitabine treatment remains controversial. These experiments were performed to evaluate the role of transcriptional vs. translational mechanisms in the ability of decitabine to increase fetal hemoglobin levels in vivo.

Materials and methods

Three normal, nonanemic baboons were treated with decitabine subcutaneously (0.5 mg/kg/d) for 10 days. The effect of decitabine on globin chain synthesis and globin messenger RNA levels was measured in pre- and posttreatment bone marrow aspirates by biosynthetic radiolabeling with [(3)H] leucine followed by separation of globin chains by high-performance liquid chromatography, and real-time polymerase chain reaction, respectively. The effect on DNA methyl

SUBMITTER: Akpan I 

PROVIDER: S-EPMC4392404 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature