Genetic susceptibility to norovirus GII.3 and GII.4 infections in Chinese pediatric diarrheal disease.

ABSTRACT:

Background

Noroviruses (NoVs) are a leading cause of viral diarrhea in young children. Secretor status has been confirmed to be linked with Norwalk virus (NoV GI.1) infection but there is limited information about whether secretor genotypes are associated with pediatric NoV epidemic strains in vivo.

Methods

In this study, fecal specimens and serum samples were collected from 124 hospitalized children with acute diarrhea in Xi'an, China. TaqMan real-time reverse transcription polymerase chain reaction was used to detect NoVs in fecal samples, and NoV-positive samples were further verified using conventional reverse transcription polymerase chain reaction and sequenced. DNA was extracted from sera and TaqMan single-nucleotide polymorphism genotyping assay was applied to determ

SUBMITTER: Liu P 

PROVIDER: S-EPMC4392756 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature