Soluble human TLR2 ectodomain binds diacylglycerol from microbial lipopeptides and glycolipids.

ABSTRACT: TLRs are key innate immune receptors that recognize conserved features of biological molecules that are found in microbes. In particular, TLR2 has been reported to be activated by different kinds of microbial ligands. To advance our understanding of the interaction of TLR2 with its ligands, the recombinant human TLR2 ectodomain (hTLR2ED) was expressed using a baculovirus/insect cell expression system and its biochemical, as well as ligand binding, properties were investigated. The hTLR2ED binds synthetic bacterial and mycoplasmal lipopeptides, lipoteichoic acid from Staphylococcus aureus, and synthetic lipoarabinomannan precursors from Mycobacterium at extracellular physiological conditions, in the absence of its co-receptors TLR1 and TLR6. We also determined that lipopeptides and glycolip

SUBMITTER: Jimenez-Dalmaroni MJ 

PROVIDER: S-EPMC4392886 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature