Quantitative PCR analysis of functional genes in iron-rich microbial mats at an active hydrothermal vent system (Lo'ihi Seamount, Hawai'i).

ABSTRACT: The chemolithotrophic Zetaproteobacteria represent a novel class of Proteobacteria which oxidize Fe(II) to Fe(III) and are the dominant bacterial population in iron-rich microbial mats. Zetaproteobacteria were first discovered at Lō'ihi Seamount, located 35 km southeast off the big island of Hawai'i, which is characterized by low-temperature diffuse hydrothermal venting. Novel nondegenerate quantitative PCR (qPCR) assays for genes associated with microbial nitrogen fixation, denitrification, arsenic detoxification, Calvin-Benson-Bassham (CBB), and reductive tricarboxylic acid (rTCA) cycles were developed using selected microbial mat community-derived metagenomes. Nitrogen fixation genes were not detected, but all other functional genes were present. This suggests that arsenic detoxificatio

SUBMITTER: Jesser KJ 

PROVIDER: S-EPMC4393452 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature