Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.

ABSTRACT:

Background and objective

Rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life-threatening.

Materials and methods

Targets IS481, IS1001, BP0026 and human GAPDH gene were used to develop a multiplex real-time PCR assay based on the TaqMan technology for detection and identification of Bordetella pertussis and Bordetella parapertussis in clinical samples. A total of 121 human clinical specimens obtained within 2012-2013 were used to evaluate the multiplex real-time PCR assay. Clinical specimens were also tested for culture and conventional PCR. Sensitivity and specificity for culture, conventional PCR, and multiplex re

SUBMITTER: Kolodkina V 

PROVIDER: S-EPMC4393489 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature