BRCA1 Alternative splicing landscape in breast tissue samples.

ABSTRACT:

Background

BRCA1 is a key protein in cell network, involved in DNA repair pathways and cell cycle. Recently, the ENIGMA consortium has reported a high number of alternative splicing (AS) events at this locus in blood-derived samples. However, BRCA1 splicing pattern in breast tissue samples is unknown. Here, we provide an accurate description of BRCA1 splicing events distribution in breast tissue samples.

Methods

BRCA1 splicing events were scanned in 70 breast tumor samples, 4 breast samples from healthy individuals and in 72 blood-derived samples by capillary electrophoresis (capillary EP). Molecular subtype was identified in all tumor samples. Splicing events were considered predominant if their relative expression level was at least the 10% of the full-length reference sig

SUBMITTER: Romero A 

PROVIDER: S-EPMC4393587 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature