TP53 R72P polymorphism modulates DNA methylation in hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is characterized by widespread epidemiological and molecular heterogeneity. Previous work showed that in the western part of North Africa, a region of low incidence of HCC, mutations are scarce for this tumor type. As epigenetic changes are considered possible surrogates to mutations in human cancers, we decided, thus, to characterize DNA methylation in HCC from North-African patients.

Methods

A set of 11 loci was investigated in a series of 45 tumor specimens using methylation-specific and combined-bisulfite restriction assay PCR. Results obtained on clinical samples were subsequently validated in liver cancer cell lines.

Results

DNA methylation at tumor suppressor loci is significantly higher in samples displaying chromosome

SUBMITTER: Rebbani K 

PROVIDER: S-EPMC4393630 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature