Novel +90G>A Intronic Polymorphism of CYP2D6.

ABSTRACT:

Objective

CYP2D6, an enzyme, metabolizes a large number of commonly prescribed drugs. Variations in CYP2D6 gene encoding this enzyme have been associated with individual differences in drug metabolism rates. The purpose of our study was to identify some allelic variants of CYP2D6 gene and to detect defective CYP2D6 alleles, as part of a pharmacogenetic screening program.

Materials and methods

A prospective study was done on 120 participants referred to Royan Institute in 2013. Allele and genotype frequencies for polymorphism of CYP2D6 gene in exons 1 and 4 were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and sequencing on PCR products, respectively.

Results

We identified a novel variant of the gene encoding cyto

SUBMITTER: Modaresi-Nejad M 

PROVIDER: S-EPMC4393675 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature