Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio).

ABSTRACT: In the present study, effect-directed analysis was used to identify teratogenic compounds in porewater collected from a Superfund site along the Elizabeth River estuary (VA, USA). Zebrafish (Danio rerio) exposed to the porewater displayed acute developmental toxicity and cardiac teratogenesis, presumably because of elevated sediment levels of polycyclic aromatic hydrocarbons (PAHs) from historical creosote use. Pretreatment of porewater with several physical and chemical particle removal methods revealed that colloid-bound chemicals constituted the bulk of the observed toxicity. Size-exclusive chromatography and normal-phase high-performance liquid chromatography were used to fractionate Elizabeth River porewater. Acute toxicity of porewater extracts and extract fractions was assessed as t

SUBMITTER: Fang M 

PROVIDER: S-EPMC4393830 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature