Polymorphic regions in the interleukin-1 gene and susceptibility to chronic periodontitis: a genetic association study.

ABSTRACT:

Objective

The objectives of this study were to determine the association between single nucleotide polymorphisms (SNPs) in IL1B (-511, +3954), IL1A (-889, +4845), and the variable number of tandem repeats (VNTRs) polymorphism in the IL-1RN gene with chronic periodontitis susceptibility and to analyze gene-gene interactions in a hospital-based sample population from South India.

Subjects and methods

A total of 400 individuals were recruited for this study; 200 individuals with healthy gingiva and 200 chronic periodontitis patients. Genomic DNA was isolated from peripheral blood samples and genotyping was performed for the above-mentioned single nucleotide and VNTR polymorphisms by polymerase chain reaction, DNA sequencing, and agarose gel electrophoresis.

Results

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SUBMITTER: Lavu V 

PROVIDER: S-EPMC4394157 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature