Structural and kinetic analysis of protein-aggregate strains in vivo using binary epitope mapping.

ABSTRACT: Despite considerable progress in uncovering the molecular details of protein aggregation in vitro, the cause and mechanism of protein-aggregation disease remain poorly understood. One reason is that the amount of pathological aggregates in neural tissue is exceedingly low, precluding examination by conventional approaches. We present here a method for determination of the structure and quantity of aggregates in small tissue samples, circumventing the above problem. The method is based on binary epitope mapping using anti-peptide antibodies. We assessed the usefulness and versatility of the method in mice modeling the neurodegenerative disease amyotrophic lateral sclerosis, which accumulate intracellular aggregates of superoxide dismutase-1. Two strains of aggregates were identified with di

SUBMITTER: Bergh J 

PROVIDER: S-EPMC4394267 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature