Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.

ABSTRACT: Fluorescence live imaging has become an essential methodology in modern cell biology. However, fluorescence requires excitation light, which can sometimes cause potential problems, such as autofluorescence, phototoxicity, and photobleaching. Furthermore, combined with recent optogenetic tools, the light illumination can trigger their unintended activation. Because luminescence imaging does not require excitation light, it is a good candidate as an alternative imaging modality to circumvent these problems. The application of luminescence imaging, however, has been limited by the two drawbacks of existing luminescent protein probes, such as luciferases: namely, low brightness and poor color variants. Here, we report the development of bright cyan and orange luminescent proteins by extending

SUBMITTER: Takai A 

PROVIDER: S-EPMC4394297 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature