C-Jun gene-modified Schwann cells: upregulating multiple neurotrophic factors and promoting neurite outgrowth.

ABSTRACT: Genetically modified Schwann cells (SCs) that overexpress neurotrophic factors (NFs), especially those that overexpress multiple NFs, hold great potential for promoting nerve regeneration. Currently, only one NF can be upregulated in most genetically modified SCs, and simultaneously upregulating multiple NFs in SCs remains challenging. In this study, we found that the overexpression of c-Jun, a component of the AP-1 transcription factor, effectively upregulated the expression and secretion of multiple NFs, including glial cell line-derived neurotrophic factor, brain-derived neurotrophic factor, artemin, leukemia inhibitory factor, and nerve growth factor. The c-Jun gene-modified SCs showed a normal morphology in scanning electron microscopy and fluorescent staining analysis. In addition, t

SUBMITTER: Huang L 

PROVIDER: S-EPMC4394874 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature