Distinct self-interaction domains promote Multi Sex Combs accumulation in and formation of the Drosophila histone locus body.

ABSTRACT: Nuclear bodies (NBs) are structures that concentrate proteins, RNAs, and ribonucleoproteins that perform functions essential to gene expression. How NBs assemble is not well understood. We studied the Drosophila histone locus body (HLB), a NB that concentrates factors required for histone mRNA biosynthesis at the replication-dependent histone gene locus. We coupled biochemical analysis with confocal imaging of both fixed and live tissues to demonstrate that the Drosophila Multi Sex Combs (Mxc) protein contains multiple domains necessary for HLB assembly. An important feature of this assembly process is the self-interaction of Mxc via two conserved N-terminal domains: a LisH domain and a novel self-interaction facilitator (SIF) domain immediately downstream of the LisH domain. Molecular mod

SUBMITTER: Terzo EA 

PROVIDER: S-EPMC4395134 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature