Genomic analysis of a Mycobacterium bovis bacillus [corrected] Calmette-Guerin strain isolated from an adult patient with pulmonary tuberculosis..

ABSTRACT: For years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A region containing the dnaA and dnaN genes that is closely related to the initial chromosome replication was found to repeat three times on the BCG Pasteur-specific tandem duplication region DU1. Due to the minimum number of epitopes in BCG strains, 3281 was inferred to have a high possibility for immune evasion. Additionally, variations in the virulence genes and predictions for potential virulence factors were ana

SUBMITTER: Li X 

PROVIDER: S-EPMC4395146 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature