Genomic location of the major ribosomal protein gene locus determines Vibrio cholerae global growth and infectivity.

ABSTRACT: The effects on cell physiology of gene order within the bacterial chromosome are poorly understood. In silico approaches have shown that genes involved in transcription and translation processes, in particular ribosomal protein (RP) genes, localize near the replication origin (oriC) in fast-growing bacteria suggesting that such a positional bias is an evolutionarily conserved growth-optimization strategy. Such genomic localization could either provide a higher dosage of these genes during fast growth or facilitate the assembly of ribosomes and transcription foci by keeping physically close the many components of these macromolecular machines. To explore this, we used novel recombineering tools to create a set of Vibrio cholerae strains in which S10-spec-α (S10), a locus bearing half of the

SUBMITTER: Soler-Bistue A 

PROVIDER: S-EPMC4395360 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature