Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants.

ABSTRACT: Nef is an accessory viral protein that promotes HIV-1 replication, facilitating alterations in cellular pathways via multiple protein-protein interactions. The advent of proteomics has expanded the focus on better identification of novel molecular pathways regulating disease progression. In this study, nef was sequenced from randomly selected patients, however, sequence variability identified did not elicited any specific mutation that could have segregated HIV-1 patients in different stages of disease progression. To explore the difference in Nef functionality based on sequence variability we used proteomics approach. Proteomic profiling was done to compare the effect of Nef variants in host cell protein expression. 2DGE in control and Nef transfected SupT1 cells demonstrated several diff

SUBMITTER: Saxena R 

PROVIDER: S-EPMC4395413 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature