Open source software for quantification of cell migration, protrusions, and fluorescence intensities.

ABSTRACT: Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by lack of access to user-friendly, automated tools. We now describe software designed for the automated quantification of cell migration and morphodynamics. Implemented as a plug-in for the open-source platform, ImageJ, ADAPT is capable of rapid, automated analysis of migration and membrane protrusions, together with associated fluorescently labeled proteins, across multiple cells. We demonstrate the ability of the software by qu

SUBMITTER: Barry DJ 

PROVIDER: S-EPMC4395480 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature