High capsid-genome correlation facilitates creation of AAV libraries for directed evolution.

ABSTRACT: Directed evolution of adeno-associated virus (AAV) through successive rounds of phenotypic selection is a powerful method to isolate variants with improved properties from large libraries of capsid mutants. Importantly, AAV libraries used for directed evolution are based on the "natural" AAV genome organization where the capsid proteins are encoded in cis from replicating genomes. This is necessary to allow the recovery of the capsid DNA after each step of phenotypic selection. For directed evolution to be used successfully, it is essential to minimize the random mixing of capsomers and the encapsidation of nonmatching viral genomes during the production of the viral libraries. Here, we demonstrate that multiple AAV capsid variants expressed from Rep/Cap containing viral genomes result in

SUBMITTER: Nonnenmacher M 

PROVIDER: S-EPMC4395795 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature