A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI.

ABSTRACT:

Background

Fragmentation at random nucleotide locations is an essential process for preparation of DNA libraries to be used on massively parallel short-read DNA sequencing platforms. Although instruments for physical shearing, such as the Covaris S2 focused-ultrasonicator system, and products for enzymatic shearing, such as the Nextera technology and NEBNext dsDNA Fragmentase kit, are commercially available, a simple and inexpensive method is desirable for high-throughput sequencing library preparation. MspJI is a recently characterised restriction enzyme which recognises the sequence motif CNNR (where R = G or A) when the first base is modified to 5-methylcytosine or 5-hydroxymethylcytosine.

Results

A semi-random enzymatic DNA amplicon fragmentation method was developed bas

SUBMITTER: Shinozuka H 

PROVIDER: S-EPMC4396059 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature