Project description:Central nervous system (CNS) projection neurons’ failure to repair/regenerate injured axons has devastating consequences for those who have sustained CNS injuries. Thus, there is a need for translatable factors capable of promoting long-distance axon regeneration in the CNS. We hypothesized that supporting lysosomes in injured neurons by supplementing their structural factors through gene therapy may foster axon regeneration. To test our hypothesis, we selected Atp6v0c for experimental regulation because it plays roles in lysosomal acidification and degradation of misfolded proteins in response to endoplasmic reticulum (ER) stress in injured neurons. We tested in a rodent optic nerve crush (ONC) model of traumatic optic neuropathy (TON) in which injured prototypical CNS projection neurons, the retinal ganglion cells (RGCs), do not regenerate damaged axons and eventually degenerate. Atp6v0c transgene expression was achieved using intravitreally-injected adeno-associated virus serotype 2 (AAV2), which transduces the RGCs. For benchmarking, we compared efficacy to AAV2-targeting of prominent regulators of axon regeneration, Pten and Klf9. We found that Atp6v0c transgene promoted RGC survival and long-distance axon regeneration, comparable to targeting Pten and Klf9. Thus, Atp6v0c is an axon regeneration-promoting factor with a potential for treating CNS injury and disease.

2026-04-09 | GSE325128 | GEO

The Retinal Ganglion Cell Repopulation, Stem Cell Transplantation, and Optic Nerve Regeneration Consortium.

Project description: Not available

| S-EPMC10630665 | biostudies-literature

Cell-free fat extract promotes axon regeneration and retinal ganglion cells survival in traumatic optic neuropathy.

Project description: Not available

| S-EPMC10954833 | biostudies-literature