Pancreatic ductal perfusion at organ procurement enhances islet yield in human islet isolation.

ABSTRACT:

Objective

Pancreas preservation is a major factor influencing the results of islet cell transplantation. This study evaluated the effects of 2 different solutions for pancreatic ductal perfusion (PDP) at organ procurement.

Methods

Eighteen human pancreases were assigned to 3 groups: non-PDP (control), PDP with ET-Kyoto solution, and PDP with cold storage/purification stock solution. Pancreatic islets were isolated according to the modified Ricordi method.

Results

No significant differences in donor characteristics, including cold ischemia time, were observed between the 3 groups. All islet isolations in the PDP groups had more than 400,000 islet equivalence in total islet yield after purification, a significant increase when compared with the control (P = 0.04 and P <

SUBMITTER: Takita M 

PROVIDER: S-EPMC4396628 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature