Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.

ABSTRACT: Palladin is a microfilament-associated phosphoprotein whose function in skeletal muscle has rarely been studied. Therefore, we investigate whether myogenesis is influenced by the depletion of palladin expression known to interfere with the actin cytoskeleton dynamic required for skeletal muscle differentiation. The inhibition of palladin in C2C12 myoblasts leads to precocious myogenic differentiation with a concomitant reduction in cell apoptosis. This premature myogenesis is caused, in part, by an accelerated induction of p21, myogenin, and myosin heavy chain, suggesting that palladin acts as a negative regulator in early differentiation phases. Paradoxically, palladin-knockdown myoblasts are unable to differentiate terminally, despite their ability to perform some initial steps of differ

SUBMITTER: Nguyen NU 

PROVIDER: S-EPMC4396843 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature