Genome-wide transcriptome and expression profile analysis of Phalaenopsis during explant browning.

ABSTRACT:

Background

Explant browning presents a major problem for in vitro culture, and can lead to the death of the explant and failure of regeneration. Considerable work has examined the physiological mechanisms underlying Phalaenopsis leaf explant browning, but the molecular mechanisms of browning remain elusive. In this study, we used whole genome RNA sequencing to examine Phalaenopsis leaf explant browning at genome-wide level.

Methodology/principal findings

We first used Illumina high-throughput technology to sequence the transcriptome of Phalaenopsis and then performed de novo transcriptome assembly. We assembled 79,434,350 clean reads into 31,708 isogenes and generated 26,565 annotated unigenes. We assigned Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KE

SUBMITTER: Xu C 

PROVIDER: S-EPMC4397044 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature