A FRET-based DNA biosensor tracks OmpR-dependent acidification of Salmonella during macrophage infection.

ABSTRACT: In bacteria, one paradigm for signal transduction is the two-component regulatory system, consisting of a sensor kinase (usually a membrane protein) and a response regulator (usually a DNA binding protein). The EnvZ/OmpR two-component system responds to osmotic stress and regulates expression of outer membrane proteins. In Salmonella, EnvZ/OmpR also controls expression of another two-component system SsrA/B, which is located on Salmonella Pathogenicity Island (SPI) 2. SPI-2 encodes a type III secretion system, which functions as a nanomachine to inject bacterial effector proteins into eukaryotic cells. During the intracellular phase of infection, Salmonella switches from assembling type III secretion system structural components to secreting effectors into the macrophage cytoplasm, enablin

SUBMITTER: Chakraborty S 

PROVIDER: S-EPMC4397060 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature