Comprehensive analysis of temporal alterations in cellular proteome of Bacillus subtilis under curcumin treatment.

ABSTRACT: Curcumin is a natural dietary compound with antimicrobial activity against various gram positive and negative bacteria. This study aims to investigate the proteome level alterations in Bacillus subtilis due to curcumin treatment and identification of its molecular/cellular targets to understand the mechanism of action. We have performed a comprehensive proteomic analysis of B. subtilis AH75 strain at different time intervals of curcumin treatment (20, 60 and 120 min after the drug exposure, three replicates) to compare the protein expression profiles using two complementary quantitative proteomic techniques, 2D-DIGE and iTRAQ. To the best of our knowledge, this is the first comprehensive longitudinal investigation describing the effect of curcumin treatment on B. subtilis proteome. The pro

SUBMITTER: Reddy PJ 

PROVIDER: S-EPMC4397091 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature