PLEKHA5 as a Biomarker and Potential Mediator of Melanoma Brain Metastasis.

ABSTRACT:

Purpose

Approximately 40% of patients with metastatic melanoma develop brain metastases. Our purpose was to identify genes aberrantly expressed in melanoma that might be associated with propensity for brain homing.

Experimental design

We studied gene expression profiles in a cell line model of brain metastasis (cerebrotropic A375Br cells vs. parental A375P cells) and compared them with profiles of patients who developed early brain metastases and who did not. A tissue microarray containing 169 metastatic melanoma cases with variable time to brain metastasis was constructed to further study marker expression by quantitative immunofluorescence. An in vitro model of the blood brain barrier (BBB) was generated to evaluate potential mediators of brain metastases.

Results

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SUBMITTER: Jilaveanu LB 

PROVIDER: S-EPMC4397107 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature