Diffuse large B-cell lymphoma classification system that associates normal B-cell subset phenotypes with prognosis.

ABSTRACT:

Purpose

Current diagnostic tests for diffuse large B-cell lymphoma use the updated WHO criteria based on biologic, morphologic, and clinical heterogeneity. We propose a refined classification system based on subset-specific B-cell-associated gene signatures (BAGS) in the normal B-cell hierarchy, hypothesizing that it can provide new biologic insight and diagnostic and prognostic value.

Patients and methods

We combined fluorescence-activated cell sorting, gene expression profiling, and statistical modeling to generate BAGS for naive, centrocyte, centroblast, memory, and plasmablast B cells from normal human tonsils. The impact of BAGS-assigned subtyping was analyzed using five clinical cohorts (treated with cyclophosphamide, doxorubicin, vincristine, and prednisone [CHOP], n

SUBMITTER: Dybkær K 

PROVIDER: S-EPMC4397280 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature