Hypoxia increases epithelial permeability in human nasal epithelia.

ABSTRACT:

Purpose

The nasal mucosa is the first site to encounter pathogens, and it forms continuous barriers to various stimuli. This barrier function is very important in the innate defense mechanism. Additionally, inflammation of the nasal sinus is known to be a hypoxic condition. Here, we studied the effect of hypoxia on barrier function in normal human nasal epithelial (NHNE) cells.

Materials and methods

The expression levels of various junction complex proteins were assessed in hypoxia-stimulated NHNE cells and human nasal mucosal tissues. We performed real-time polymerase chain reaction analysis, western blotting, and immunofluorescence assays to examine differences in the mRNA and protein expression of ZO-1, a tight junction protein, and E-cadherin in NHNE cells. Moreover, we

SUBMITTER: Min HJ 

PROVIDER: S-EPMC4397456 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature