Optimizing nanodiscs and bicelles for solution NMR studies of two β-barrel membrane proteins.

ABSTRACT: Solution NMR spectroscopy has become a robust method to determine structures and explore the dynamics of integral membrane proteins. The vast majority of previous studies on membrane proteins by solution NMR have been conducted in lipid micelles. Contrary to the lipids that form a lipid bilayer in biological membranes, micellar lipids typically contain only a single hydrocarbon chain or two chains that are too short to form a bilayer. Therefore, there is a need to explore alternative more bilayer-like media to mimic the natural environment of membrane proteins. Lipid bicelles and lipid nanodiscs have emerged as two alternative membrane mimetics that are compatible with solution NMR spectroscopy. Here, we have conducted a comprehensive comparison of the physical and spectroscopic behavior o

SUBMITTER: Kucharska I 

PROVIDER: S-EPMC4397663 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature