Targeted single molecule sequencing methodology for ovarian hyperstimulation syndrome.

ABSTRACT:

Background

One of the most significant issues surrounding next generation sequencing is the cost and the difficulty assembling short read lengths. Targeted capture enrichment of longer fragments using single molecule sequencing (SMS) is expected to improve both sequence assembly and base-call accuracy but, at present, there are very few examples of successful application of these technologic advances in translational research and clinical testing. We developed a targeted single molecule sequencing (T-SMS) panel for genes implicated in ovarian response to controlled ovarian hyperstimulation (COH) for infertility.

Results

Target enrichment was carried out using droplet-base multiplex polymerase chain reaction (PCR) technology (RainDance®) designed to yield amplicons averaging

SUBMITTER: Orkunoglu-Suer F 

PROVIDER: S-EPMC4397691 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature