Proteogenomics analysis reveals specific genomic orientations of distal regulatory regions composed by non-canonical histone variants.

ABSTRACT:

Background

Histone variants play further important roles in DNA packaging and controlling gene expression. However, our understanding about their composition and their functions is limited.

Results

Integrating proteomic and genomic approaches, we performed a comprehensive analysis of the epigenetic landscapes containing the four histone variants H3.1, H3.3, H2A.Z, and macroH2A. These histones were FLAG-tagged in HeLa cells and purified using chromatin immunoprecipitation (ChIP). By adopting ChIP followed by mass spectrometry (ChIP-MS), we quantified histone post-translational modifications (PTMs) and histone variant nucleosomal ratios in highly purified mononucleosomes. Subsequent ChIP followed by next-generation sequencing (ChIP-seq) was used to map the genome-wide localiza

SUBMITTER: Won KJ 

PROVIDER: S-EPMC4397702 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature