A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis.

ABSTRACT:

Background

Genes and signalling pathways involved in pluripotency have been studied extensively in mouse and human pre-implantation embryos and embryonic stem (ES) cells. The unsuccessful attempts to generate ES cell lines from other species including cattle suggests that other genes and pathways are involved in maintaining pluripotency in these species. To investigate which genes are involved in bovine pluripotency, expression profiles were generated from morula, blastocyst, trophectoderm and inner cell mass (ICM) samples using microarray analysis. As MAPK inhibition can increase the NANOG/GATA6 ratio in the inner cell mass, additionally blastocysts were cultured in the presence of a MAPK inhibitor and changes in gene expression in the inner cell mass were analysed.

Results

SUBMITTER: Brinkhof B 

PROVIDER: S-EPMC4397860 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature