Non-enzymatic glycation alters microdamage formation in human cancellous bone.

ABSTRACT:

Introduction

The accumulation of advanced glycation end-products (AGEs) in bone has been suggested to adversely affect the fracture resistance of bone with aging, diabetes, and pharmacological treatments. The formation of AGEs increases crosslinking in the organic matrix of bone but it is unknown how elevated levels of AGEs affect the mechanisms of fracture resistance such as microdamage formation.

Methods

Human tibial cancellous bone cores were subjected to non-enzymatic glycation (NEG) by in vitro ribosylation and were mechanically loaded to pre- (0.6%) and post- (1.1%) yield apparent level strains. Loaded specimens were stained with lead-uranyl acetate and subjected to microCT-based 3D quantification and characterization of microdamage as either diffuse damage and linear

SUBMITTER: Tang SY 

PROVIDER: S-EPMC4398022 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature