Technical Advance: Transcription factor, promoter, and enhancer utilization in human myeloid cells.

ABSTRACT: The generation of myeloid cells from their progenitors is regulated at the level of transcription by combinatorial control of key transcription factors influencing cell-fate choice. To unravel the global dynamics of this process at the transcript level, we generated transcription profiles for 91 human cell types of myeloid origin by use of CAGE profiling. The CAGE sequencing of these samples has allowed us to investigate diverse aspects of transcription control during myelopoiesis, such as identification of novel transcription factors, miRNAs, and noncoding RNAs specific to the myeloid lineage. We further reconstructed a transcription regulatory network by clustering coexpressed transcripts and associating them with enriched cis-regulatory motifs. With the use of the bidirectional expressi

SUBMITTER: Joshi A 

PROVIDER: S-EPMC4398258 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature