Enhancement of dynamin polymerization and GTPase activity by Arc/Arg3.1.

ABSTRACT:

Background

The Activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Its role in endocytosis may be mediated by its reported interaction with dynamin 2, a 100 kDa GTPase that polymerizes around the necks of budding vesicles and catalyzes membrane scission.

Methods

Enzymatic and turbidity assays are used in this study to monitor effects of Arc on dynamin activity and polymerization. Arc oligomerization is measured using a combination of approaches, including size exclusion chromatography, sedimentation analysis, dynamic light scattering, fluorescence correlation spectrosco

SUBMITTER: Byers CE 

PROVIDER: S-EPMC4398645 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature