Down-regulation of PTEN expression due to loss of promoter activity in human hepatocellular carcinoma cell lines.

ABSTRACT:

Aim

To investigate the regulation of phosphatase and tensin homolog deleted on chromosome ten (PTEN) gene expression in human hepatocellular carcinoma (HCC) cell lines.

Methods

The mRNA and protein levels of PTEN were detected by Northern blot and Western blot in HCC cell lines, respectively. Plasmids containing different fragments of PTEN promoter with Luciferase reporter were constructed and transiently transfected into HCC cell lines to study the promoter activity. DNA analysis and RT-PCR were performed to detect the mutation of PTEN promoter and PTEN cDNA.

Results

Either protein or mRNA levels of PTEN in L02 cells (as a control) were significantly higher than that in HCC cell lines. The profile of PTEN promoter activity in 8 cell lines was closely correlated with

SUBMITTER: Ma DZ 

PROVIDER: S-EPMC4398694 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature