Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli.

ABSTRACT:

Background

The Gram-negative bacterium Escherichia coli has been widely used as a cell factory for the production of proteins and specialty chemicals because it is the best characterized host with many available expression and regulation systems. However, recombinant proteins produced in Escherichia coli are generally intracellular and often found in the form of inclusion bodies. Extracellular production of proteins is advantageous compared with intracellular production because extracellular proteins can be purified more easily and can avoid protease attack, which results in higher product quality. In this study, we found a catalytic domain of a cellulase (Cel-CD) and its N-terminus can be employed as carriers for extracellular production of recombinant proteins.

Results

In

SUBMITTER: Gao D 

PROVIDER: S-EPMC4399388 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature