Antibiotic treatment suppresses rotavirus infection and enhances specific humoral immunity.

ABSTRACT:

Background

Rotavirus causes 500 000 deaths and millions of physician visits and hospitalizations per year, with worse outcomes and reduced vaccine efficacy in developing countries. We hypothesized that the gut microbiota might modulate rotavirus infection and/or antibody response and thus potentially play a role in such regional differences.

Methods

The microbiota was ablated via germ-free or antibiotic approaches. Enhanced exposure to microbiota was achieved via low-dose dextran sodium sulfate (DSS) treatment. Rotavirus infection and replication was assessed by enzyme-linked immunosorbent assay (ELISA) and quantitative reverse-transcription polymerase chain reaction. Diarrhea was scored visually. Humoral responses to rotavirus were measured by ELISA and enzyme-linked immuno

SUBMITTER: Uchiyama R 

PROVIDER: S-EPMC4399425 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature