Titration of biologically active amyloid-β seeds in a transgenic mouse model of Alzheimer's disease.

ABSTRACT: Experimental evidence in animal models suggests that misfolded Amyloid-β (Aβ) spreads in disease following a prion-like mechanism. Several properties characteristics of infectious prions have been shown for the induction of Aβ aggregates. However, a detailed titration of Aβ misfolding transmissibility and estimation of the minimum concentration of biologically active Aβ seeds able to accelerate pathological changes has not yet been performed. In this study, brain extracts from old tg2576 animals were serially diluted and intra-cerebrally injected into young subjects from the same transgenic line. Animals were sacrificed several months after treatment and brain slices were analyzed for amyloid pathology. We observed that administration of misfolded Aβ was able to significantly accelerate am

SUBMITTER: Morales R 

PROVIDER: S-EPMC4399520 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature