Differential transcriptional profiling of damaged and intact adjacent dorsal root ganglia neurons in neuropathic pain.

ABSTRACT: Neuropathic pain, caused by a lesion in the somatosensory system, is a severely impairing mostly chronic disease. While its underlying molecular mechanisms are not thoroughly understood, neuroimmune interactions as well as changes in the pain pathway such as sensitization of nociceptors have been implicated. It has been shown that not only are different cell types involved in generation and maintenance of neuropathic pain, like neurons, immune and glial cells, but, also, intact adjacent neurons are relevant to the process. Here, we describe an experimental approach to discriminate damaged from intact adjacent neurons in the same dorsal root ganglion (DRG) using differential fluorescent neuronal labelling and fluorescence-activated cell sorting (FACS). Two fluorescent tracers, Fluoroemerald

SUBMITTER: Reinhold AK 

PROVIDER: S-EPMC4400143 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature