Comparative evaluation of three commercial quantitative cytomegalovirus standards by use of digital and real-time PCR.

ABSTRACT: The recent development of the 1st WHO International Standard for human cytomegalovirus (CMV) and the introduction of commercially produced secondary standards have raised hopes of improved agreement among laboratories performing quantitative PCR for CMV. However, data to evaluate the trueness and uniformity of secondary standards and the consistency of results achieved when these materials are run on various assays are lacking. Three concentrations of each of the three commercially prepared secondary CMV standards were tested in quadruplicate by three real-time and two digital PCR methods. The mean results were compared in a pairwise fashion with nominal values provided by each manufacturer. The agreement of results among all methods for each sample and for like concentrations of each stan

SUBMITTER: Hayden RT 

PROVIDER: S-EPMC4400765 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature