Surface enhanced Raman spectroscopy detection of biomolecules using EBL fabricated nanostructured substrates.

ABSTRACT: Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, a

SUBMITTER: Peters RF 

PROVIDER: S-EPMC4401373 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature