A versatile click-compatible monolignol probe to study lignin deposition in plant cell walls.

ABSTRACT: Lignin plays important structural and functional roles in plants by forming a hydrophobic matrix in secondary cell walls that enhances mechanical strength and resists microbial decay. While the importance of the lignin matrix is well documented and the biosynthetic pathways for monolignols are known, the process by which lignin precursors or monolignols are transported and polymerized to form this matrix remains a subject of considerable debate. In this study, we have synthesized and tested an analog of coniferyl alcohol that has been modified to contain an ethynyl group at the C-3 position. This modification enables fluorescent tagging and imaging of this molecule after its incorporation into plant tissue by click chemistry-assisted covalent labeling with a fluorescent azide dye, and conf

SUBMITTER: Pandey JL 

PROVIDER: S-EPMC4401456 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature