A combined light sheet fluorescence and differential interference contrast microscope for live imaging of multicellular specimens.

ABSTRACT: We describe a microscope capable of both light sheet fluorescence microscopy and differential interference contrast microscopy (DICM). The two imaging modes, which to the best of our knowledge have not previously been combined, are complementary: light sheet fluorescence microscopy provides three-dimensional imaging of fluorescently labelled components of multicellular systems with high speed, large fields of view, and low phototoxicity, whereas differential interference contrast microscopy reveals the unlabelled neighbourhood of tissues, organs, and other structures with high contrast and inherent optical sectioning. Use of a single Nomarski prism for differential interference contrast microscopy and a shared detection path for both imaging modes enables simple integration of the two tech

SUBMITTER: Baker RP 

PROVIDER: S-EPMC4401621 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature