Analyzing internal fragmentation of electrosprayed ubiquitin ions during beam-type collisional dissociation.

ABSTRACT: Gaseous fragmentation of intact proteins is multifaceted and can be unpredictable by current theories in the field. Contributing to the complexity is the multitude of precursor ion states and fragmentation channels. Terminal fragment ions can be re-fragmented, yielding product ions containing neither terminus, termed internal fragment ions. In an effort to better understand and capitalize upon this fragmentation process, we collisionally dissociated the high (13+), middle (10+), and low (7+) charge states of electrosprayed ubiquitin ions. Both terminal and internal fragmentation processes were quantified through step-wise increases of voltage potential in the collision cell. An isotope fitting algorithm matched observed product ions to theoretical terminal and internal fragment ions. At op

SUBMITTER: Durbin KR 

PROVIDER: S-EPMC4401654 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature