Dataset Information
Identification of Entamoeba histolytica by Molecular Method in Surface Water of Rasht City, Iran.
Ontology highlight
ABSTRACT: Background
This study was conducted with the aim of determining surface water contamination with cysts of Entamoeba histolytica using PCR in Rasht City, Northern Iran.Methods
In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012. After filtration using 0.22 μm nitrate cellulose membrane filters, the samples were examined using microscope and PCR method.Results
In microscopic examination, four samples of the 49 samples were positive for cysts of E. (histolytica / dispar / muschkovskii). By using PCR method and molecular analysis, one sample was positive for E. histolytica.Conclusion
In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City. Further investigations including more samples and necessary preparations must be applied to prevent contamination.
SUBMITTER: Hemmati A
PROVIDER: S-EPMC4401882 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Publications
Identification of Entamoeba histolytica by Molecular Method in Surface Water of Rasht City, Iran.
Iranian journal of public health 20150201 2
<h4>Background</h4>This study was conducted with the aim of determining surface water contamination with cysts of Entamoeba histolytica using PCR in Rasht City, Northern Iran.<h4>Methods</h4>In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012. After filtration using 0.22 μm nitrate cellulose membrane filters, the samples were examined using microscope and PCR method.<h4>Results</h4>In mi ...[more]
