Identification of distinct circulating exosomes in Parkinson's disease.

ABSTRACT:

Objective

Whether circulating microvesicles convey bioactive signals in neurodegenerative diseases remains currently unknown. In this study, we investigated the biochemical composition and biological function of exosomes isolated from sera of patients with Parkinson's disease (PD).

Methods

Proteomic analysis was performed on microvesicle preparations from grouped samples of patients with genetic and sporadic forms of PD, amyotrophic lateral sclerosis, and healthy subjects. Nanoparticle-tracking analysis was used to assess the number and size of exosomes between patient groups. To interrogate their biological effect, microvesicles were added to primary rat cortical neurons subjected to either nutrient deprivation or sodium arsenite.

Results

Among 1033 proteins identifi

SUBMITTER: Tomlinson PR 

PROVIDER: S-EPMC4402081 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature